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Learn Bioinformatics Data Analysis - Master Python, Linux & R - OneDDL - 12-24-2024
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Free Download Learn Bioinformatics Data Analysis - Master Python, Linux & R
Published: 11/2024
Created by: Rafiq Ur Rehman
MP4 | Video: h264, 1280x720 | Audio: AAC, 44.1 KHz, 2 Ch
Level: Beginner | Genre: eLearning | Language: English | Duration: 38 Lectures ( 7h 17m ) | Size: 3.43 GB
Master Data Analysis for Bioinformatics: Python, Linux & R for RNA-Seq and Genomic Data Using Hands-on Practical's
What you'll learn
Practical, Hands-On Learning: You'll gain the skills to process and analyze biological data in real-world scenarios using Python, Linux, and R.
Master Basic Python Programming: Learn fundamental Python concepts, including variables, loops, and conditionals, and apply them in the context of bioinformatic
Utilize Biopython for Sequence Operations: Gain hands-on experience with Biopython to manipulate and analyze biological sequences (DNA, RNA, protein).
Work with FASTQ Files: Learn how to process, filter, and analyze FASTQ files commonly used in sequencing data, with a focus on quality control.
Access and Query Biological Databases: Use Biopython to interact with public biological databases (e.g., NCBI, GenBank) for retrieving and analyzing genomic dat
Perform Sequence Alignment in Python: Understand the basics of sequence alignment and apply algorithms for pairwise and multiple sequence alignments using Biopy
Implement BLAST with Python: Learn how to programmatically use the BLAST tool through Biopython for sequence comparison and searching against sequence databases
Visualize Biological Data: Use Python libraries (such as Matplotlib and Seaborn) to visualize genomic data, protein structures, and sequence alignments.
Manipulate Large Biological Data Files: Learn to index, sort, and filter large biological data files using Python to ensure efficient analysis.
Manage and Process Biological Data on Linux: Develop proficiency in using Linux for bioinformatics, including file management, text processing, and command-line
Master Bash Scripting for Bioinformatics Workflows: Learn how to write Bash scripts to automate common bioinformatics tasks such as data preprocessing, alignmen
Implement Bioinformatics Pipelines: Understand how to design and implement bioinformatics pipelines on Linux, including steps for data retrieval, processing, an
Preprocess RNA-Seq Data: Learn how to preprocess raw RNA-Seq data, including quality control, trimming, and removing adapter sequences.
Perform RNA-Seq Alignment and Quantification: Align RNA-Seq data to a reference genome and perform expression quantification using tools like STAR, HISAT2, and
Analyze Differential Gene Expression: Use R to perform differential expression analysis on RNA-Seq data, identify significantly expressed genes, and interpret r
Conduct Gene Set Enrichment Analysis (GSEA): Learn to perform GSEA using R to analyze functional categories and biological pathways associated with gene express
Requirements
Beginner-friendly: This course is designed to teach Python, Linux, and R from the ground up. You don't need prior programming knowledge to get started.
The course will include foundational lessons on Python programming, so even if you're new to coding, you'll be able to follow along.
Understanding basic concepts like percentages, averages, and standard deviation will be helpful for some aspects of data analysis (e.g., differential gene expression).
No advanced math or statistical knowledge is required; the course will focus on practical applications of these concepts.
Familiarity with using a computer, navigating directories, and managing files.
Basic understanding of operating systems (Windows, macOS, or Linux).
No prior bioinformatics experience is required. The course is designed to guide you through essential bioinformatics tools and workflows.
Even if you're new to programming or bioinformatics, the step-by-step lessons will introduce you to the basic concepts and help you build confidence in your skills.
Support and Guidance: You'll have access to clear explanations, resources, and troubleshooting tips throughout the course, which are designed to support beginners.
Description
In today's rapidly evolving world of genomics, bioinformatics, and computational biology, the ability to analyze and interpret vast amounts of biological data has become a crucial skill. From sequencing genomes to studying gene expression, the tools and techniques in bioinformatics are essential for answering complex biological questions and advancing research. If you're interested in learning how to leverage computational methods for biological analysis, this course is designed for you."Learn Bioinformatics Data Analysis: Master Python, Linux & R" is a comprehensive, hands-on course designed to teach you the foundational skills and techniques needed to process, analyze, and visualize genomic data. Whether you're an aspiring bioinformatician, a life science student, or a researcher looking to enhance your data analysis capabilities, this course will provide you with the tools and knowledge necessary to succeed in the world of bioinformatics.This course takes a multi-faceted approach by teaching you three key components: Python programming, Linux operating systems, and R for bioinformatics. These are the core tools that drive bioinformatics workflows today, and by mastering them, you'll gain the ability to handle everything from basic sequence analysis to complex RNA-Seq data analysis.What you will learn:Throughout this course, you will:Master Python for Bioinformatics:Learn Python from the ground up, starting with basic concepts like variables, loops, and conditionals, and moving towards advanced bioinformatics applications.Use the Biopython library to work with biological data, including DNA, RNA, and protein sequences.Understand how to read and manipulate FASTQ files, perform sequence alignment, and use the BLAST tool programmatically.Explore various data formats in bioinformatics, including FASTA, GFF, and VCF, and learn how to process these formats efficiently.Learn to visualize biological data with Python, using libraries like Matplotlib and Seaborn, to create insightful visualizations of genomic sequences and alignments.Become Proficient in Linux for Bioinformatics:Master the basics of the Linux command line, an essential skill for anyone working in bioinformatics.Learn to navigate the Linux file system, manage files and directories, and automate tasks using Bash scripting.Understand how to install and manage bioinformatics tools and software packages in a Linux environment, a common setup in computational biology labs.Learn how to retrieve data from biological databases using command-line tools, such as wget and curl, and process this data efficiently.Gain Expertise in RNA-Seq Data Analysis Using Linux
ive deep into RNA-Seq, one of the most powerful techniques in genomics today, and learn the entire pipeline from data preprocessing to alignment and quantification.Learn how to preprocess RNA-Seq data, including quality control, trimming, and adapter removal using tools like FastQC and Trimmomatic.Understand how to align RNA-Seq reads to a reference genome using alignment tools like STAR or HISAT2, and quantify gene expression using featureCounts or HTSeq.Learn how to interpret the results from RNA-Seq analysis, identify differentially expressed genes, and visualize the outcomes using tools such as DESeq2 and edgeR.Utilize R for Bioinformatics:Learn how to set up R and RStudio for bioinformatics data analysis, and explore the data structures in R such as vectors, matrices, and data frames.Learn to work with bioinformatics datasets in R, including importing, cleaning, and manipulating biological data.Master the use of R for statistical analysis in bioinformatics, including performing differential expression analysis and Gene Set Enrichment Analysis (GSEA).Visualize genomic data using ggplot2, a powerful visualization package in R, to create plots like volcano plots, heatmaps, and PCA plots that can help in interpreting gene expression data.Course Structure:The course is divided into four major sections, each covering a specific area of bioinformatics and computational biology. Here's what you can expect from each section:Section 1: Python for BioinformaticsThis section covers the basics of Python and introduces Biopython, a library specifically designed for bioinformatics applications. By the end of this section, you will be able to manipulate biological sequences, work with various data formats, and perform tasks like sequence alignment and BLAST searches using Python.Key Topics:Introduction to Python programmingBasic sequence operations with BiopythonWorking with FASTQ files and filtering dataSequence alignment algorithms and toolsPerforming BLAST searches with BiopythonVisualizing genomic data with PythonSection 2: Linux for BioinformaticsLinux is the backbone of most bioinformatics workflows. In this section, you'll learn to navigate the Linux file system, use command-line tools to manipulate data, and automate bioinformatics tasks with Bash scripts.Key Topics:Introduction to Linux and basic commandsNavigating the Linux file systemText file manipulation and data extractionInstalling and managing bioinformatics tools on LinuxScripting with Bash to automate bioinformatics tasksRetrieving biological data from online databasesSection 3: RNA-Seq Data Analysis Using LinuxThis section focuses on RNA-Seq, a widely used method to study gene expression. You will learn how to preprocess RNA-Seq data, align it to a reference genome, and quantify gene expression using various bioinformatics tools.Key Topics:Introduction to RNA-Seq and its applicationsQuality control of RNA-Seq data using FastQCPreprocessing RNA-Seq data with FastpAligning RNA-Seq data using BwaQuantifying gene expression with featureCountsVisualizing differential expression resultsSection 4: R for BioinformaticsIn this section, you'll gain expertise in using R for bioinformatics data analysis. You'll learn how to visualize, analyze, and interpret large biological datasets, including RNA-Seq data, and conduct statistical analyses to derive meaningful insights.Key Topics:Setting up R and RStudioData manipulation and analysis in RDifferential gene expression analysis with DESeq2 and edgeRVisualizing genomic data using ggplot2Performing Gene Set Enrichment Analysis (GSEA) in RWho Is This Course For?This course is ideal for anyone interested in bioinformatics and data science, including:Aspiring bioinformaticians looking to break into the field of genomics and computational biology.Biology and life science students who want to gain computational skills for data analysis.Researchers and scientists involved in genomics, transcriptomics, or proteomics who need to process large biological datasets.Data analysts and statisticians wanting to expand their skill set into bioinformatics.You don't need prior programming or bioinformatics experience to start this course, as it is designed to accommodate beginners. However, a basic understanding of biology and genetics will help you contextualize the data you'll be working with.Why Take This Course?Comprehensive Learning: This course covers the essential tools and techniques needed for bioinformatics data analysis. By the end, you'll be proficient in Python, Linux, and R-three of the most widely used tools in bioinformatics.Hands-On Projects: Throughout the course, you will work on real-world bioinformatics projects and datasets, which will help you gain practical experience in the field.Career Advancement: Bioinformatics is a rapidly growing field with applications in medicine, biotechnology, and environmental science. By mastering these skills, you'll position yourself for opportunities in research, academia, and industry.Beginner-Friendly: Even if you have no prior experience in programming or bioinformatics, this course will guide you step by step through the essential concepts and tools.Enroll now and take your first step toward mastering bioinformatics data analysis using Python, Linux, and R!Who this course is for
Individuals who want to break into the field of bioinformatics and learn how to analyze biological data using Python, Linux, and R.
Those who are looking to gain practical skills in sequence analysis, RNA-Seq data, and genomic research tools.
Undergraduate or graduate students in biology, genetics, molecular biology, or related fields who want to enhance their computational skills for bioinformatics research.
Students who need to understand how to work with biological datasets, genomic sequences, and high-throughput sequencing technologies.
Whether you're a beginner looking to explore the field or someone who wants to add a new dimension to their data analysis toolkit, this course will provide the foundational knowledge and hands-on skills needed to succeed.
People from diverse academic or professional backgrounds who want to develop practical skills in bioinformatics and computational biology.
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